Composite
Ω

Part:BBa_K2300001:Design

Designed by: Ari Edmonds   Group: iGEM17_Macquarie_Australia   (2017-10-03)


Hydrogen Gas Producing Gene Cluster


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1792
    Illegal NheI site found at 5180
    Illegal NheI site found at 5390
    Illegal NheI site found at 5834
    Illegal NotI site found at 3584
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4232
    Illegal NgoMIV site found at 8188
    Illegal AgeI site found at 5147
    Illegal AgeI site found at 6790
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6015
    Illegal BsaI.rc site found at 3635
    Illegal BsaI.rc site found at 3747


Design Notes

Removal of introns and optimization of codons.


Source

Chlamydomonas reinhardtii

References